Эрдэм шинжилгээ, судалгааны салбар
Spermatology & Andrology
  • Sperm Induction of acrosome reaction in spermatozoa accelates development of fertilized human oocyte after intracytoplasmic sperm injection. (52th ASRM, 1996).
  • Identification of round spermatid from testis biopsy and its application in human ART program. (10th World Congress on IVF & ART, 1997)
  • Detailed semen analysis and ICSI in azoospermia-like infertile patients. (53th ASRM, 1997)
  • AZF and Microdeletion Microdeletions of Y Chromosome in Korean Male Infertility Patients (5th ICA,1997)
  • Evaluation of spermatogenesis by DAZ and protamine-2 gene in human testis biopsy (5th ICA, 1997)
  • The Incidence of microdeletion and the frequency of AZFa, b, c deletion in male infertility patients. (53th ASRM, 1997)
  • ART Application of ICSI or ROSI by testicular biopsy in non-obstructive azoospermia.(53th ASRM, 1997)
  • Analysis of fertilization, embryonic development and pregnancy rates after ICSI using spermatozoa obtained from fresh or frozen-thawed testicular tissues (54th ASRM, 1998)
  • Sperm DNA fragmentation assay: Comet assay (CERM 2011),
  • Halosperm assay (KSRM 2012),
  • Sperm protamine deficiency assay: CMA3 assay and Aniline blue assay (KSRM 2012)
Oogenesis and Folliculogenesis
  • Oocytes Production of mature oocyte from frozen-thawed primordial follicular oocyte by reconstruction with cytoplasm of full grown germinal vesicle(GV)-stage oocyte. (55th ASRM, 1999)
  • Follicle In vitro Growth of Primordial Follicular Oocyte in the Frozen-Thawed Ovarian Tissue of Neonatal Mouse (54th ASRM, 1998)
  • Steroidogenesis in the Frozen-Thawed 18 Weeks Human Fetal Ovarian Tissues Cultured In Vitro (54th ASRM, 1998)
  • TGF βI and TGF βII were detected from fresh 18 weeks fetal ovarian tissue in human.
  • Ovarian Endocrinology Expression of Melatonin receptor gene in mouse gonad. (54th ASRM, 1998)
  • The synergistic effect of VIP and FSH in vitro on the expression of FSH receptor gene in human fetal ovary. (55th ASRM, 1999)
  • Expression of receptor genes for gonadotrophins and estrogens, and biosynthesis of estrogen in mid-gestational stage of human ovarian tissues. (55th ASRM, 1999)
  • Expression and localization of cell cycle-related proteins and receptor genes for gonadotropins, estrogen, and aromatase in human fetal ovaries; a preliminary study (International Workshop on Early Folliculogenesis and Oocyte Development,1999)
  • The effect of vasoactive intestinal peptide on estradiol biosynthesis in human fetal ovarian tissue in vitro.(International Workshop on Early Folliculogenesis and Oocyte Development 1999)
Embryogenesis, Hatching and Implantation
  • Embryology Regulation of embryonic genome activation and compaction by the transfer of cytoplasm transfer with inhibitors of protein synthesis or protein kinase in mouse. (32th SSR, 1999)
  • Hatching Biochemical Assisted Hatching by Proteases Increased Pregnancy Rates in Human IVF-ET Program (1996' ASRM)
  • Application of biochemically assisted hatching(BAH) by proteases in human assisted reproductive technology program. (53th ASRM, 1997)
  • Improvement of pregnancy and implantation rate by biochemical assisted hatching in human IVF-ET. (11th World Congress on IVF & Hum Reprod Genet, 1999)
  • Outcome of Biochemical Assisted Hatching in 1068 IVF-ET Cycles Using Pronase in Human Assisted Reproductive Technology. (11th World Congress on IVF & Hum Reprod Genet, 1999)
  • Biochemical assisted hatching(BAH) increased the implantation and pregnancy rate in human cryopreserved embryo transfer. (55h ASRM, 1999)
  • Implantation Regulation of integrin a1, av, and b3 gene expression by leukemia inhibitory factor(LIF) during blastocyst outgrowth in the mouse.(30th SSR, 1997)
ART
  • Hatching and outgrowth according to various freezing procedures in mouse blastocyst cryopreservation. (12th ESHRE, 1996)
  • Pregnancy from Translocation carrier patient with recurrent abortion by preimplantation genetic diagnosis using fluorescence in-situ hybridization. (52th ASRM, 1996)
  • Assisted Reproductive Technology Program by Immature Oocyte Retrieval in Non-stimulated Cycle (54th ASRM, 1998)
  • Clinical use of immature oocyte from normal and PCO women in non-stimulated IVF-ET program. (55th ASRM, 1999)
  • Fragmentation of embryos is associated with both necrosis and apoptosis. (Fertil Steril 2011).
ROS (Reactive Oxygen Species)
  • Effect of the antioxidant supplemented into semen or sperm washing medium on functional parameter of human spermatozoa (KSFS 2006, Hum Reprod 2008)
  • Correlation between seminal parameter of patients and sperm DNA fragmentation estimated by comet assay (KSFS 2006, CERM 2011)
  • Detection of oxidative stress on sperm by using Oxisperm kit
Stem cells
Adult stem cells
  • In vitro proliferation and differentiation of placenta derived cell and HSC isolated from umbilical cord blood
  • Effect of oxygen tension and antioxidant on proliferation of HSC in vitro
Embryonic stem cells
  • Establishment of gene knock-in system in hES cells
  • Single cell culture of hES cells
  • Differentiation of hES cells into germ cells
Genetics
Cytogenetics
  • Amniotic fluid
  • Chorionic villi cells
  • Peripheral blood/ Cord blood
  • Tissue
Molecular genetics
  • Preimplantation genetic diagnosis (PGD, FISH)
  • Preimplantation genetic screening (PGS, FISH)
  • Azoospermia related gene test (Daz test)
  • Amniotic fluid FISH (18, 21)